Journal: Metabolic Brain Disease

Article Title: Adropin ameliorates behavioral seizures and the relevant neuroinflammation, oxidative stress, and neural damage in a rat model of pentylenetetrazole-induced seizure potentially by reducing the activation of NF-κB/IkB-α signaling pathway

doi: 10.1007/s11011-025-01654-2

Figure Lengend Snippet: Antioxidant and neuroprotective effects of adropin in the cortex in PTZ-induced epileptic conditions. Effects of adropin and other drug administrations on the levels of MDA ( A ), SOD1 ( B ), GFAP ( C ) and BDNF ( D ) in the cortex. AD1, 2 µg/kg dose of adropin; AD2, 10 µg/kg dose of adropin; LN, L-NAME; PTZ, pentylenetetrazole; VPA, valproic acid. * p < 0.05, ** p < 0.01 and *** p < 0.001

Article Snippet: ELISA kits for rat interleukin (IL)−1β, TNF-α, brain-derived neurotrophic factor (BDNF), glial fibrillary acidic protein (GFAP), malondialdehyde (MDA), and superoxide dismutase-1 (SOD1) were purchased from Elabscience (Houston, Texas, USA).

Techniques:

Journal: Metabolic Brain Disease

Article Title: Adropin ameliorates behavioral seizures and the relevant neuroinflammation, oxidative stress, and neural damage in a rat model of pentylenetetrazole-induced seizure potentially by reducing the activation of NF-κB/IkB-α signaling pathway

doi: 10.1007/s11011-025-01654-2

Figure Lengend Snippet: Antioxidant and neuroprotective effects of adropin in the hippocampus in PTZ-induced epileptic conditions. Effects of adropin and other drug administrations on the levels of MDA ( A ), SOD1 ( B ), GFAP ( C ) and BDNF ( D ) in the hippocampus. AD1, 2 µg/kg dose of adropin; AD2, 10 µg/kg dose of adropin; LN, L-NAME; PTZ, pentylenetetrazole; VPA, valproic acid. * p < 0.05, ** p < 0.01 and *** p < 0.001

Article Snippet: ELISA kits for rat interleukin (IL)−1β, TNF-α, brain-derived neurotrophic factor (BDNF), glial fibrillary acidic protein (GFAP), malondialdehyde (MDA), and superoxide dismutase-1 (SOD1) were purchased from Elabscience (Houston, Texas, USA).

Techniques:

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Impact of adropin on superoxide dismutase expression in pancreatic β‐cells of normoglycemic and diabetic rats. (A) Immunofluorescence labeling with anti‐superoxide dismutase antibody and anti‐insulin antibodies showed expression of superoxide dismutase in pancreatic β‐cells. (B) Quantification of the histological analysis showed a significant (*** p < 0.001) decrease in superoxide dismutase distribution in pancreatic endocrine cells and a significant (**** p < 0.0001) decrease in its localization in β‐cells of rats with diabetes when compared to the normal group. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Labeling

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on superoxide dismutase expression in pancreatic α‐cells of normal and diabetic rats. (A) Immunofluorescence staining using anti‐superoxide dismutase and anti‐glucagon antibodies showed expression of superoxide dismutase in pancreatic α‐cells. (B) Quantification of the histological analysis showed a significant (** p < 0.01) decrease in superoxide dismutase distribution in the pancreatic endocrine cells of diabetic rats compared to the normal group. α‐Cell expression of superoxide dismutase did not change with adropin treatment among all groups. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on catalase, superoxide dismutase and total glutathione activities in the serum samples of healthy and diabetic rats. (A) Catalase activity was significantly decreased in diabetic rats compared to normal controls. Adropin slightly increased catalase in DMT group. (B) Superoxide dismutase was slightly increased in DMT compared to the diabetic treated with adropin. (C) Total glutathione was significantly raised with adropin treatment in DMT compared to DMUT. n = 4–6. Data analysis was done using the ANOVA test. * p < 0.05.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Activity Assay

Journal: Current Issues in Molecular Biology

Article Title: The Effects of Engeletin on Insulin Resistance Induced in Human HepG2 Liver Cells

doi: 10.3390/cimb47070535

Figure Lengend Snippet: ( A ) . The analysis results of the effect of Engeletin on MDA, ( B ) GSH, and ( C ) SOD (ENG: Engeletin; IR: insulin resistance; MET: metformin). *: p < 0.05.

Article Snippet: Oxidant and antioxidant parameters—malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) levels—were measured using human-specific ELISA kits (Wuhan, China) (MDA: ELABSCIENCE (E-BC-K025-M); GSH: ELABSCIENCE(E-EL-H5410); and SOD: ELABSCIENCE (E-EL-H1113)) with two repetitions.

Techniques:

Journal: Neuroscience letters

Article Title: A potential role for neuronal connexin 36 in the pathogenesis of amyotrophic lateral sclerosis

doi: 10.1016/j.neulet.2017.12.027

Figure Lengend Snippet: Knockdown of Cx36 prevents neuronal death caused by overexpression of the SOD1-G93A mutant protein. Statistical data from MTT assay (A) and representative images from western blot experiments (B, C) in WT mouse purified neuronal spinal cord cultures are shown. The cultures were transduced with shRNA-LUC or shRNA-Cx36 and also were transfected with the empty vector (VECT) or plasmids inducing human SOD1 or G93A mutant. shRNA-Cx36 effectively suppressed Cx36 protein expression (B). Cultures transfected with the SOD1 and G93A mutant demonstrated the FLAG reactivity (C). Statistical analysis in A: ANOVA with post hoc Tukey; n = 8 per group in all groups; *P<0.05; NS, non-significant.

Article Snippet: An expression plasmid for (Myc-DDK-tagged)-human soluble SOD1 was purchased from Origene, Inc. (Rockville, MD, USA; cat.# RC200725; Accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_000454.4","term_id":"48762945","term_text":"NM_000454.4"}} NM_000454.4 ).

Techniques: Knockdown, Over Expression, Mutagenesis, MTT Assay, Western Blot, Purification, Transduction, shRNA, Transfection, Plasmid Preparation, Expressing